Journal: Biochemistry and Biophysics Reports

Article Title: Inhibition of FGFR signaling attenuates phosphaturia and early kidney injury in sickle cell disease mice

doi: 10.1016/j.bbrep.2026.102671

Figure Lengend Snippet: Schematic illustration of signaling pathways involved in BGJ398-mediated improvement in dysregulated phosphate metabolism in the kidneys of SCD mice. In SCD, anemia stimulates the kidneys to secrete erythropoietin (EPO), which travels to bone and triggers fibroblast growth factor 23 (FGF23) production. High level of FGF23 activate FGFR1/αKlotho signaling in the kidneys, leading to phosphaturia. Increased FGFR signaling in the kidneys also promotes inflammation. Inhibition of FGFR signaling with BGJ398 alleviates phosphate wasting and improves renal phosphate handling in SCD mice.

Article Snippet: The reagents used in this study, including the control IgG and the anti-FGF23 antibody (clone 58.5) kindly provided by Amgen Inc. (Thousand Oaks, CA).

Techniques: Protein-Protein interactions, Inhibition

Journal: Biology

Article Title: DMP1-Mediated FAK Activation Contributes to P Utilization of Broiler Osteoblasts by Suppressing FGF23 Expression

doi: 10.3390/biology15020121

Figure Lengend Snippet: The effect of si-DMP1 or pcDNA3.1-DMP1 transfection on the mRNA expression levels of DMP1 ( A , D ), FAK ( B , E ), and FGF23 ( C , F ) in broiler osteoblasts. All values are expressed as means ± SE, n = 5–6. * p < 0.05, ** p < 0.01, *** p < 0.001. DMP1, dentin matrix protein 1; FAK, focal adhesion kinase; FGF23, fibroblast growth factor 23.

Article Snippet: After being blocked in a 5% BSA or skim milk solution for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies against DMP1 (at 1:500 dilution; Abclonal, Wuhan, China), FLAG (at 1:5000 dilution; Mabnus, Wuhan, China), FAK (at 1: 500 dilution; BD Biosciences, San Jose, CA, USA), phosphorylated (p-) FAK (at 1:1000 dilution; Abcam, Cambridge, UK), FGF23 (at 1:500 dilution; Abclonal, Wuhan, China), and β-actin (at 1:5000 dilution; Huaxingbio, Beijing, China), followed by the incubation with goat anti-rabbit or anti-mouse secondary antibody (at 1:5000 dilution; Huaxingbio, Beijing, China).

Techniques: Transfection, Expressing

Journal: Biology

Article Title: DMP1-Mediated FAK Activation Contributes to P Utilization of Broiler Osteoblasts by Suppressing FGF23 Expression

doi: 10.3390/biology15020121

Figure Lengend Snippet: The effect of si-DMP1 or pcDNA3.1-DMP1 transfection on the protein expression levels of DMP1 ( A , E ), FAK ( B , F ), p-FAK ( C , G ), FGF23 ( D , H ), and FGF23 content ( I , J ) in broiler osteoblasts. All values are expressed as means ± SE, n = 4–6. * p < 0.05, ** p < 0.01, *** p < 0.001. DMP1, dentin matrix protein 1; FAK, focal adhesion kinase; p-FAK, phosphorylated FAK; FGF23, fibroblast growth factor 23.

Article Snippet: After being blocked in a 5% BSA or skim milk solution for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies against DMP1 (at 1:500 dilution; Abclonal, Wuhan, China), FLAG (at 1:5000 dilution; Mabnus, Wuhan, China), FAK (at 1: 500 dilution; BD Biosciences, San Jose, CA, USA), phosphorylated (p-) FAK (at 1:1000 dilution; Abcam, Cambridge, UK), FGF23 (at 1:500 dilution; Abclonal, Wuhan, China), and β-actin (at 1:5000 dilution; Huaxingbio, Beijing, China), followed by the incubation with goat anti-rabbit or anti-mouse secondary antibody (at 1:5000 dilution; Huaxingbio, Beijing, China).

Techniques: Transfection, Expressing

Journal: Biology

Article Title: DMP1-Mediated FAK Activation Contributes to P Utilization of Broiler Osteoblasts by Suppressing FGF23 Expression

doi: 10.3390/biology15020121

Figure Lengend Snippet: The effect of si-FGF23 or pcDNA3.1-FGF23 transfection on FGF23 mRNA expression ( A , D ), FGF23 protein expression ( B , E ), and FGF23 content ( C , F ) in broiler osteoblasts. All values are expressed as means ± SE, n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001. FGF23, fibroblast growth factor 23.

Article Snippet: After being blocked in a 5% BSA or skim milk solution for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies against DMP1 (at 1:500 dilution; Abclonal, Wuhan, China), FLAG (at 1:5000 dilution; Mabnus, Wuhan, China), FAK (at 1: 500 dilution; BD Biosciences, San Jose, CA, USA), phosphorylated (p-) FAK (at 1:1000 dilution; Abcam, Cambridge, UK), FGF23 (at 1:500 dilution; Abclonal, Wuhan, China), and β-actin (at 1:5000 dilution; Huaxingbio, Beijing, China), followed by the incubation with goat anti-rabbit or anti-mouse secondary antibody (at 1:5000 dilution; Huaxingbio, Beijing, China).

Techniques: Transfection, Expressing

Journal: Biology

Article Title: DMP1-Mediated FAK Activation Contributes to P Utilization of Broiler Osteoblasts by Suppressing FGF23 Expression

doi: 10.3390/biology15020121

Figure Lengend Snippet: The effects of FGF23 silencing and overexpression on the P utilization rate ( A , E ), ARS concentration ( B , F ), ALP activity ( C , G ) and BGP content ( D , H ) in broiler osteoblasts. All values are expressed as means ± SE, n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001. FGF23, fibroblast growth factor 23; ARS, alizarin red S; ALP, alkaline phosphatase activity; BGP, bone gla protein. ARS concentration (mmol/L) was used to represent the mineralization formation.

Article Snippet: After being blocked in a 5% BSA or skim milk solution for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies against DMP1 (at 1:500 dilution; Abclonal, Wuhan, China), FLAG (at 1:5000 dilution; Mabnus, Wuhan, China), FAK (at 1: 500 dilution; BD Biosciences, San Jose, CA, USA), phosphorylated (p-) FAK (at 1:1000 dilution; Abcam, Cambridge, UK), FGF23 (at 1:500 dilution; Abclonal, Wuhan, China), and β-actin (at 1:5000 dilution; Huaxingbio, Beijing, China), followed by the incubation with goat anti-rabbit or anti-mouse secondary antibody (at 1:5000 dilution; Huaxingbio, Beijing, China).

Techniques: Over Expression, Concentration Assay, Activity Assay

Journal: Biology

Article Title: DMP1-Mediated FAK Activation Contributes to P Utilization of Broiler Osteoblasts by Suppressing FGF23 Expression

doi: 10.3390/biology15020121

Figure Lengend Snippet: FAK overexpression decreased FGF23 expression and production in broiler osteoblasts. ( A ) FAK mRNA. ( B ) Total FAK protein. ( C ) p-FAK protein. ( D ) FGF23 mRNA. ( E ) FGF23 protein. ( F ) FGF23 content. All values are expressed as means ± SE, n = 4–6. * p < 0.05, ** p < 0.01, *** p < 0.001. FAK, focal adhesion kinase; p-FAK, phosphorylated FAK; FGF23, fibroblast growth factor 23.

Article Snippet: After being blocked in a 5% BSA or skim milk solution for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies against DMP1 (at 1:500 dilution; Abclonal, Wuhan, China), FLAG (at 1:5000 dilution; Mabnus, Wuhan, China), FAK (at 1: 500 dilution; BD Biosciences, San Jose, CA, USA), phosphorylated (p-) FAK (at 1:1000 dilution; Abcam, Cambridge, UK), FGF23 (at 1:500 dilution; Abclonal, Wuhan, China), and β-actin (at 1:5000 dilution; Huaxingbio, Beijing, China), followed by the incubation with goat anti-rabbit or anti-mouse secondary antibody (at 1:5000 dilution; Huaxingbio, Beijing, China).

Techniques: Over Expression, Expressing

Journal: Biology

Article Title: DMP1-Mediated FAK Activation Contributes to P Utilization of Broiler Osteoblasts by Suppressing FGF23 Expression

doi: 10.3390/biology15020121

Figure Lengend Snippet: Attenuated FAK weakened the inhibitory effect of DMP1 on FGF23 expression in broiler osteoblasts. ( A ) FAK mRNA. ( B ) Total FAK protein. ( C ) p-FAK protein. ( D ) FGF23 mRNA. ( E ) FGF23 protein. ( F ) FGF23 content. All values are expressed as means ± SE, n = 3–6. Lacking the same letters (a–c) means differences at p < 0.05. DMP1, dentin matrix protein 1; FAK, focal adhesion kinase; p-FAK, phosphorylated FAK; FGF23, fibroblast growth factor 23.

Article Snippet: After being blocked in a 5% BSA or skim milk solution for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies against DMP1 (at 1:500 dilution; Abclonal, Wuhan, China), FLAG (at 1:5000 dilution; Mabnus, Wuhan, China), FAK (at 1: 500 dilution; BD Biosciences, San Jose, CA, USA), phosphorylated (p-) FAK (at 1:1000 dilution; Abcam, Cambridge, UK), FGF23 (at 1:500 dilution; Abclonal, Wuhan, China), and β-actin (at 1:5000 dilution; Huaxingbio, Beijing, China), followed by the incubation with goat anti-rabbit or anti-mouse secondary antibody (at 1:5000 dilution; Huaxingbio, Beijing, China).

Techniques: Expressing